This week as I mentioned before was the first patient of the prostate clinical trial and the prostate obtained from surgery was imaged for identifying the PSMA+ loci of the cancer. The day started in the OR where I witnessed the robotic prostectomy performed with the help of Davinci robotic surgery equipment. I was able to tell the advantage of using a robot because the size of the incision was smaller than a regular surgery and since the patient just had a Gleason 7 tumor he was a candidate for robotic prostectomy. The excised prostate was taken to the pathology lab where the anterior-posterior of the prostate was marked and feducials were inserted. Then the excised prostate was imaged using MRI and PET-CT. The Scherenkov imaging was done using in vivo imager. We were able to see the localization of antibody to the tumor alone in the entire prostate.
As far my project is concerned, I was working with the grad student in Osborne's lab on sorting prostate cancer cells transfected with vectors for expressing PSMA and GFP. Initially we are planning to test for successful transfection using flow cytometry and then sort them using FACS.
No comments:
Post a Comment